Articles

Interaction of Laser and Adenosine Triphosphate (ATP) Molecule

Introduction: The light is absorbed by specific and non-specific chromophores, like melanin and metallo-prosthetic groups in proteins. As some authors founded response to the light in situations, where any known chromophores was present. The aim of this study was to demonstrate that other structures, and more concretely, the high-energy phosphor-oxygen bond of ATP molecule, ca absorb light.

Method and Material: We used three different diode lasers (635, 655 and 830 nm) at different irradiances and fluence (15-30 mW/cm², and 1 to 6 J/cm²).
The first system we studied was the luciferine-luciferase reaction that’s commonly used to check ATP behavior.
The second one, the phosphorylation of the glucose by glucokinase, is an important cell reaction that needs ARP to occur.
All results were statistically treated with Student-T, Anova and Bonferroni.

Results: In both systems we got strong changes between irradiated and non-irradiated ATP and specially significance with 830 nm. Irradiated one increased the kinetics of the reaction.
We conclude that the light-excited form of ATP has different biochemical behavior than non-excited ones.