Articles

In Vitro Exposure of Wounded Diabetic Fibroblast Cells to a Helium-Neon Laser at 5 and 16J/cm2

In Vitro Exposure of Wounded Diabetic Fibroblast Cells to a Helium-Neon Laser at 5 and 16 J/cm²

Houreld, N., Abrahamse, H.
Photomedicine and Laser Surgery 2007
25)2): 78-84

Objective: The aim of the present investigation was to assess morphological, cellular, and molecular effects of exposing wounded diabetic fibroblast cells to He-Ne (632.8nm) laser irradiation at two different doses.

Background Data: An alternative treatment modality for diabetic wound healing includes low-level laser therapy (LLLT). Although it’s used in many countries and for many medical conditions, too many health care workers are unaware of this therapy, and there is still controversy surrounding its effectiveness.

Methods: Normal human skin fibroblast cells (WS1) were used to simulate a wounded diabetic model. The effect of LLLT (632.8nm, 5 and 16 J/cm² once a day on two non-consecutive days) was determined by analysis of cell morphology, cytotoxicity, apoptosis, and DNA damage.

Results: Cells exposed to 5 J/cm² showed a higher rate of migration than cells exposed to 16J/cm², and there was a complete wound closure by day 4. Exposure of WS1 cells to 5J/cm² on two non-consecutive days did not induce additional cytotoxicity of genetic damage, whereas exposure to 16 J/cm² did. There was a significant increase in apoptosis in exposed cells as compared to unexposed cells.

Conclusion: Based on cellular morphology, exposure to 5J/cm² was stimulatory to cell migration, whereas exposure to 16J/cm² was inhibitory. Exposure to 16J/cm² induced genetic damage on WS1 cells when exposed to a He-Ne laser in vitro, whereas exposure to 5J/cm² did not induced any additional damage.

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